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Protein names :SuperSignal Enhancer Chemiluminescent (ECL plus) Substrate,发光液,ECL
Form :Liquid
Reactivity :Human,Rat,Mouse
Function :SuperSignal Enhancer Chemiluminescent (ECL )Substrate is a sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. SuperSignal Enhancer Chemiluminescent (ECL) Substrate enables low femtogram detection of antigen by oxidizing luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence that can be visualized on X-ray film or an imaging system. Optimal signal intensity and duration can be attained with appropriate primary and secondary antibody dilutions (see Below Table).
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.