Enhancer Chemiluminescent (ECL) SubstrateCat NO.: TA0050

Protein names ：Enhancer Chemiluminescent (ECL) Substrate

Form ：Liquid

Reactivity ：Human,Rat,Mouse

• ApplicationDilution
• 免疫印迹(WB)1：1
• The optimal dilutions should be determined by the end user

Function ：Enhancer Chemiluminescent (ECL )Substrate is a sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots.SuperSignal Enhancer Chemiluminescent (ECL) Substrate enables low femtogram detection of antigen by oxidizing luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence that can be visualized on X-ray film or an imaging system. Optimal signal intensity and duration can be attained with appropriate primary and secondary antibody dilutions (see Below Table).

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.