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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using 14-3-3 γ Rabbit mAb [4I45] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :YWHAG,1433G_HUMAN,14-3-3 protein gamma [Cleaved into: 14-3-3 protein gamma, N-terminally processed]
UniProtID :P61981
MASS(da) :28,303
MW(kDa) :27 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human 14-3-3 γ
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol.Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner..
Tissue specificity :Highly expressed in brain, skeletal muscle, and heart..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.