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Western blot(SDS PAGE) analysis of extracts from PC12 cell lysate.Using Tyrosine Hydroxylase Rabbit mAb [69MU]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EC 1.14.16.2; TH isoform 3; TH isoform a; TH-4; TY3H; TYH; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase; tyrosine hydroxylase;
UniProtID :P07101
MASS(da) :58,600
MW(kDa) :58kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Tyrosine Hydroxylase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :PC12 cell lysate.
Function : Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (PubMed:17391063, PubMed:1680128, PubMed:15287903, PubMed:8528210, Ref.18, PubMed:34922205, PubMed:24753243). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity).., [Isoform 5]: Lacks catalytic activity.., [Isoform 6]: Lacks catalytic activity..
Tissue specificity :Mainly expressed in the brain and adrenal glands.
Subcellular locationi :Cytoplasm, perinuclear region. Nucleus. Cell projection, axon. Cytoplasm. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.