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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using pro Caspase 10 Rabbit mAb [8tw6]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Apoptotic protease Mch4; CASP10; Caspase 10; FLICE2; ICE like apoptotic protease 4; FADD-like ICE2;
UniProtID :Q92851
MASS(da) :58,951
MW(kDa) :59kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human Mouse
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human pro Caspase 10
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.., Isoform 7 can enhance NF-kappaB activity but promotes only slight apoptosis.., Isoform C is proteolytically inactive..
Tissue specificity :Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.