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HNF-4-alpha Rabbit mAb [14iD]Cat NO.: A52693

Western blot(SDS PAGE) analysis of extracts from A549 cell lysate.Using HNF-4-alpha Rabbit mAb [14iD]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :NR2A1; HNF4A; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; Transcription factor 14; TCF-14; Transcription factor HNF-4; HNF4; TCF14; Nuclear receptor subfamily 2 group A member 1;

UniProtID :P41235

MASS(da) :52,785

MW(kDa) :53kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human HNF-4-alpha

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A549 cell lysate.

Function : Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes (PubMed:30597922). Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells (PubMed:30530698)..

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.