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EpCAM Rabbit mAb [b1Qz]Cat NO.: A92974

Western blot(SDS PAGE) analysis of extracts from A431 cell lysate.Using EpCAM Rabbit mAb [b1Qz]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :Epithelial cell adhesion molecule; Ep-CAM; CO17A; CD326; Cell surface glycoprotein Trop-1; EGP; Epithelial glycoprotein 314; EGP314; hEGP314; KS 1/4 antigen; KSA;

UniProtID :P16422

MASS(da) :34,932

MW(kDa) :35kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human EpCAM

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A431 cell lysate.

Function : May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E..

Tissue specificity :Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma..

Subcellular locationi :Lateral cell membrane,Single-pass type I membrane protein. Cell junction, tight junction.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.