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Western blot(SDS PAGE) analysis of extracts from A431 cell lysate.Using EpCAM Rabbit mAb [b1Qz]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Epithelial cell adhesion molecule; Ep-CAM; CO17A; CD326; Cell surface glycoprotein Trop-1; EGP; Epithelial glycoprotein 314; EGP314; hEGP314; KS 1/4 antigen; KSA;
UniProtID :P16422
MASS(da) :34,932
MW(kDa) :35kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human EpCAM
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A431 cell lysate.
Function : May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E..
Tissue specificity :Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma..
Subcellular locationi :Lateral cell membrane,Single-pass type I membrane protein. Cell junction, tight junction.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.