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Western blot(SDS PAGE) analysis of extracts from A431 cell lysate.Using Cytokeratin 4 Rabbit mAb [KJAr]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Keratin4; Cytokeratin 4; Keratin-4; CK-4; CK4; KRT14; CYK4;
UniProtID :P19013
MASS(da) :56,144
MW(kDa) :57kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Cytokeratin 4
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A431 cell lysate.
Tissue specificity :Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.