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Cytokeratin 4 Rabbit mAb [KJAr]Cat NO.: A47195

Western blot(SDS PAGE) analysis of extracts from A431 cell lysate.Using Cytokeratin 4 Rabbit mAb [KJAr]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :Keratin4; Cytokeratin 4; Keratin-4; CK-4; CK4; KRT14; CYK4;

UniProtID :P19013

MASS(da) :56,144

MW(kDa) :57kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Cytokeratin 4

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A431 cell lysate.

Tissue specificity :Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer..

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.