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P-Cyclin E1 (T77) Rabbit mAb [7EO8]Cat NO.: A16624

Western blot(SDS PAGE) analysis of extracts from (1) JAR cell treated with Lambda Phosphatase lysate; (2) JAR cell lysate.Using P-Cyclin E1 (T77) Rabbit mAb [7EO8]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CCNE; Ccne1; cyclin E variant ex5del; cyclin E variant ex7del; Cyclin E1; Cyclin Es; Cyclin Et; G1/S specific cyclin E; G1/S-specific cyclin-E1;

UniProtID :P24864

MASS(da) :47,077

MW(kDa) :48kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Cyclin E1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :(1) JAR cell treated with Lambda Phosphatase lysate; (2) JAR cell lysate.

Function : Essential for the control of the cell cycle at the G1/S (start) transition..

Tissue specificity :Highly expressed in testis and placenta. Low levels in bronchial epithelial cells..

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.