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Western blot(SDS PAGE) analysis of extracts from A172 cells treated with aphidicolin (10 μg/mL 24 hr) .Using PAF15 Rabbit mAb [10HJ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PCLAF,KIAA0101,NS5ATP9,PAF,L5,PAF15_HUMAN,PCNA-associated factor
UniProtID :Q15004
MASS(da) :11,986
MW(kDa) :15 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human PAF15
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A172 cells treated with aphidicolin (10 μg/mL 24 hr)
Function : PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number..
Tissue specificity :Expressed predominantly in liver, pancreas and placenta. Not detected in heart or brain. Highly expressed in a number of tumors, especially esophageal tumors, in anaplastic thyroid carcinomas, adrenocortical carcinomas, and in non-small-cell lung cancer lines..
Subcellular locationi :Nucleus. Cytoplasm, perinuclear region.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.