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Western blot(SDS PAGE) analysis of extracts from (1) HeLa cell lysate; (2) 3T3 cell lysate; (3) C6 cell lysate.Using STAT5b Rabbit mAb [Yw2m]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Signal transducer and activator of transcription 5B; STA5B; STAT5; Stat5b; Transcription factor STAT5B;
UniProtID :P51692
MASS(da) :89,866
MW(kDa) :90kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human STAT5b
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) HeLa cell lysate; (2) 3T3 cell lysate; (3) C6 cell lysate.
Function : Carries out a dual signal transduction and activation of transcription (PubMed:29844444). Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation..
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.