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P-JunD (S255) Rabbit mAb [04J6]Cat NO.: A62360

Western blot(SDS PAGE) analysis of extracts from (1) HeLa cell lysate treated with Alkaline Phosphatase; (2) HeLa cell lysate.Using P-JunD (S255) Rabbit mAb [04J6]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :Activator protein 1; AP 1; Jun D; jun D proto oncogene; Jund; JunD FL isoform; Transcription factor jun D;

UniProtID :P17535

MASS(da) :35,174

MW(kDa) :38,42kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human JunD

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :(1) HeLa cell lysate treated with Alkaline Phosphatase; (2) HeLa cell lysate.

Function : Transcription factor binding AP-1 sites (PubMed:9989505). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to an AP-1 consensus sequence 3'-TGA[GC]TCA-5' and enhancing their transcriptional activity (PubMed:9989505, PubMed:28981703)..

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.