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Western blot(SDS PAGE) analysis of extracts from Hela cell lysate treated with hydroxyurea.Using Cdc6 Rabbit mAb [NrL8]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CDC6; CDC18L; CDC6-related protein; Cdc18-related protein; HsCDC6; HsCDC18; p62(cdc6);
UniProtID :Q99741
MASS(da) :62,720
MW(kDa) :63kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Cdc6
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Hela cell lysate treated with hydroxyurea.
Function : Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Subcellular locationi :Nucleus. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.