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Cdc6 Rabbit mAb [NrL8]Cat NO.: A99400

Western blot(SDS PAGE) analysis of extracts from Hela cell lysate treated with hydroxyurea.Using Cdc6 Rabbit mAb [NrL8]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CDC6; CDC18L; CDC6-related protein; Cdc18-related protein; HsCDC6; HsCDC18; p62(cdc6);

UniProtID :Q99741

MASS(da) :62,720

MW(kDa) :63kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Cdc6

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Hela cell lysate treated with hydroxyurea.

Function : Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.

Subcellular locationi :Nucleus. Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.