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Western blot(SDS PAGE) analysis of extracts from K562 cell lysate.Using Cdc25C Rabbit mAb [g453]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CDC25; CDC25C; Cell division cycle 25 homolog C; MPIP3; PPP1R60;
UniProtID :P30307
MASS(da) :53,365
MW(kDa) :60kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Cdc25C
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K562 cell lysate.
Function : Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.