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Western blot(SDS PAGE) analysis of extracts from mouse skeletal muscle lysate.Using Hsp20 Rabbit mAb [V9JL]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HSPB6; HEL55; Hsp20; Heat-shock 20 kDa like-protein p20;
UniProtID :O14558
MASS(da) :17,136
MW(kDa) :17kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Hsp20
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :mouse skeletal muscle lysate.
Function : Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Seems to have versatile functions in various biological processes. Plays a role in regulating muscle function such as smooth muscle vasorelaxation and cardiac myocyte contractility. May regulate myocardial angiogenesis implicating KDR. Overexpression mediates cardioprotection and angiogenesis after induced damage. Stabilizes monomeric YWHAZ thereby supporting YWHAZ chaperone-like activity..
Subcellular locationi :Cytoplasm. Nucleus. Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.