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Western blot(SDS PAGE) analysis of extracts from MCF7 cell lysate.Using CDCA3 Rabbit mAb [U5rs]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Cell division cycle-associated protein 3; Trigger of mitotic entry protein 1; TOME-1; Gene-rich cluster protein C8; CDCA3; C8; GRCC8; TOME1;
UniProtID :Q99618
MASS(da) :28,998
MW(kDa) :26kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human CDCA3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF7 cell lysate.
Function : F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase (By similarity)..
Subcellular locationi :Cytoplasm, cytosol.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.