support:13376277882 | tech@naturebios.com

PBEF/NAMPT Rabbit mAb [A83E]Cat NO.: A34097

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using PBEF/NAMPT Rabbit mAb [A83E] at dilution of 1:1000 incubated at 4℃ over night.

  • SizePriceIn stock
  • 50ul1280咨询客服
  • 100ul2380咨询客服
Order Datasheet

Product information

Protein names :NAMPT,PBEF,PBEF1,NAMPT_HUMAN,Nicotinamide phosphoribosyltransferase

UniProtID :P43490

MASS(da) :55,521

MW(kDa) :52 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human PBEF/NAMPT

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity)..

Tissue specificity :Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.

Subcellular locationi :Nucleus. Cytoplasm. Secreted.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.