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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using Hsp47 Rabbit mAb [626G]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2;
UniProtID :P50454
MASS(da) :46,441
MW(kDa) :46kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Hsp47
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Subcellular locationi :Endoplasmic reticulum lumen.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.