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A-RAF Rabbit mAb [LA0D]Cat NO.: A30641

Western blot(SDS PAGE) analysis of extracts from A375 cell lysate.Using A-RAF Rabbit mAb [LA0D]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :ARAF; A-raf-1; PKS2; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Ras-binding protein DA-Raf; Oncogene ARAF1; ARAF1; RAFA1;

UniProtID :P10398

MASS(da) :67,585

MW(kDa) :68kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human A-RAF

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A375 cell lysate.

Function : Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.., [Isoform 2]: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation..

Tissue specificity :Predominantly in urogenital tissues.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.