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Western blot(SDS PAGE) analysis of extracts from A375 cell lysate.Using A-RAF Rabbit mAb [LA0D]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ARAF; A-raf-1; PKS2; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Ras-binding protein DA-Raf; Oncogene ARAF1; ARAF1; RAFA1;
UniProtID :P10398
MASS(da) :67,585
MW(kDa) :68kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human A-RAF
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A375 cell lysate.
Function : Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.., [Isoform 2]: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation..
Tissue specificity :Predominantly in urogenital tissues.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.