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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using Mitofilin Rabbit mAb [i13D]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Cell proliferation-inducing gene 4/52 protein; Heart muscle protein; HMP; IMMT; p87/89; pig4; PIG52;
UniProtID :Q16891
MASS(da) :83,678
MW(kDa) :84kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Mitofilin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology (PubMed:22114354, PubMed:25781180)..
Subcellular locationi :Mitochondrion inner membrane,Single-pass membrane protein. Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.