Caspase-10 Rabbit mAb [8Kl8]Cat NO.: A92773

Protein names ：Apoptotic protease Mch4; CASP10; Caspase10; FLICE2; ICE like apoptotic protease 4; FADD-like ICE2;

UniProtID ：Q92851

MASS(da) ：58,951

MW(kDa) ：59kDa

Form ：Liquid

Purification ：Affinity-chromatography

Host ：Rabbit

Isotype ： IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with A synthesized peptide derived from human Caspase-10

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：Jurkat cell lysate.

Function ： Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.., Isoform 7 can enhance NF-kappaB activity but promotes only slight apoptosis.., Isoform C is proteolytically inactive..

Tissue specificity ：Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.