TIMP1 Rabbit mAb [VQ2V]Cat NO.: A95813

Protein names ：CLGI; Collagenase inhibitor; EPA; EPO; HCI; Metalloproteinase inhibitor 1; TIMP; TIMP1;

UniProtID ：P01033

MASS(da) ：23,171

MW(kDa) ：26kDa

Form ：Liquid

Purification ：Affinity-chromatography

Host ：Rabbit

Isotype ： IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with A synthesized peptide derived from human TIMP1

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：HL60 cell lysate treated with TPA.

Function ： Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro,but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors..

Tissue specificity ：Detected in rheumatoid synovial fluid (at protein level)..

Subcellular locationi ：Secreted.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.