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Western blot(SDS PAGE) analysis of extracts from human fetal skin lysate.Using Collagen X Rabbit mAb [1woP]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CollagenX; COL10A1; COAA1; Collagen type X alpha 1;
UniProtID :Q03692
MASS(da) :66,158
MW(kDa) :66kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Collagen X
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human fetal skin lysate.
Function : Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
Subcellular locationi :Secreted, extracellular space, extracellular matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.