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Western blot(SDS PAGE) analysis of extracts from SW480 cell lysate.Using HE4 Rabbit mAb [criI]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :WFDC2; Epididymis-specific he4; Epididymal protein 4; DJ461P17.6; EDDM4; HE4; WAP5;
UniProtID :Q14508
MASS(da) :12,993
MW(kDa) :23kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human Mouse
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human HE4
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SW480 cell lysate.
Function : Broad range protease inhibitor..
Tissue specificity :Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis..
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.