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Western blot(SDS PAGE) analysis of extracts from (1) A431 cell lysate; (2) RAW264.7 cell lysate.Using S100A10 Rabbit mAb [d8Tv]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Protein S100-A10; Calpactin I light chain; Calpactin-1 light chain; S100A10; ANX2LG; CAL1L; CLP11;
UniProtID :P60903
MASS(da) :11,203
MW(kDa) :11kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human Mouse
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human S100A10
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) A431 cell lysate; (2) RAW264.7 cell lysate.
Function : Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.