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Western blot(SDS PAGE) analysis of extracts from HUVEC cell lysate.Using CD99 Rabbit mAb [uK6S]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CD99 antigen; 12E7; E2 antigen; Protein MIC2; CD99; MIC2; MIC2X; MIC2Y;
UniProtID :P14209
MASS(da) :18,848
MW(kDa) :28kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human CD99
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HUVEC cell lysate.
Function : Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity)..
Subcellular locationi :Membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.