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CD99 Rabbit mAb [uK6S]Cat NO.: A53357

Western blot(SDS PAGE) analysis of extracts from HUVEC cell lysate.Using CD99 Rabbit mAb [uK6S]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CD99 antigen; 12E7; E2 antigen; Protein MIC2; CD99; MIC2; MIC2X; MIC2Y;

UniProtID :P14209

MASS(da) :18,848

MW(kDa) :28kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human CD99

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HUVEC cell lysate.

Function : Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity)..

Subcellular locationi :Membrane,Single-pass type I membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.