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Western blot(SDS PAGE) analysis of extracts from human fetal liver lysate.Using 58K Golgi protein Rabbit mAb [CdN4]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Formimidoyltetrahydrofolate cyclodeaminase; FTCD; Glutamate formiminotransferase; LCHC 1;
UniProtID :O95954
MASS(da) :58,927
MW(kDa) :58kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human 58K Golgi protein
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human fetal liver lysate.
Function : Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.., Binds and promotes bundling of vimentin filaments originating from the Golgi..
Subcellular locationi :Cytoplasm, cytosol. Golgi apparatus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.