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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using PDHA1 Rabbit mAb [848M]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Pyruvate dehydrogenase E1 component subunit alpha, somatic form; mitochondrial; PDHE1-A type I; PDHA1; PHE1A;
UniProtID :P08559
MASS(da) :43,296
MW(kDa) :43kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human PDHA1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle..
Tissue specificity :Ubiquitous.
Subcellular locationi :Mitochondrion matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.