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Western blot(SDS PAGE) analysis of extracts from human brain lysate.Using NEFH Rabbit mAb [JkjI]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NF-H; 200 kDa neurofilament protein; Neurofilament triplet H protein; KIAA0845; NFH; NEFH;
UniProtID :P12036
MASS(da) :112,479
MW(kDa) :200kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human NEFH
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human brain lysate.
Function : Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity)..
Subcellular locationi :Cytoplasm, cytoskeleton. Cell projection, axon.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.