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NEFH Rabbit mAb [JkjI]Cat NO.: A27450

Western blot(SDS PAGE) analysis of extracts from human brain lysate.Using NEFH Rabbit mAb [JkjI]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :NF-H; 200 kDa neurofilament protein; Neurofilament triplet H protein; KIAA0845; NFH; NEFH;

UniProtID :P12036

MASS(da) :112,479

MW(kDa) :200kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human NEFH

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :human brain lysate.

Function : Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity)..

Subcellular locationi :Cytoplasm, cytoskeleton. Cell projection, axon.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.