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Western blot(SDS PAGE) analysis of extracts from SH-SY5Y cell lysate.Using Ras Rabbit mAb [kxQA]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GTPase HRas; GTPase KRas; GTPase NRas; H-Ras-1; H-RASIDX; Ha-Ras; HAMSV; HRAS; K RAS2A; K RAS2B; K RAS4A; K RAS4B; K-RAS; KRAS; N-RAS; N-terminally processed; NRAS; p21ras; RASH1; RASK2;
UniProtID :P01111/P01112/P01116
MASS(da) :21,229
MW(kDa) :22kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Ras
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SH-SY5Y cell lysate.
Function : Ras proteins bind GDP/GTP and possess intrinsic GTPase activity..
Subcellular locationi :Cell membrane,Lipid-anchor,Cytoplasmic side. Golgi apparatus membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.