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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using TGN46 Rabbit mAb [L7xK]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TGN38; TGN46; TGN48; TGN51; TGOLN2; TTGN2;
UniProtID :O43493
MASS(da) :45,880
MW(kDa) :90kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human TGN46
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : May be involved in regulating membrane traffic to and from trans-Golgi network.
Tissue specificity :Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.