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Western blot(SDS PAGE) analysis of extracts from human serum lysate.Using ApoB Rabbit mAb [GYh8]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ApoB 100; ApoB 48; Apolipoprotein B 100; Apolipoprotein B48; FLDB;
UniProtID :P04114
MASS(da) :515,605
MW(kDa) :515kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human ApoB
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human serum lysate.
Function : Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Subcellular locationi :Cytoplasm. Secreted. Lipid droplet.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.