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Actin Rabbit mAb [Q1py]Cat NO.: A13861

Western blot(SDS PAGE) analysis of extracts from (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.Using Actin Rabbit mAb [Q1py]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :ACTA; ACTA1; Actin alpha skeletal muscle; Alpha Actin 1;alpha-actin; Alpha-actin-1; ASMA; CFTD; CFTD1; CFTDM;

UniProtID :P68133

MASS(da) :42,051

MW(kDa) :42kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Actin

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :(1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.

Function : Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subcellular locationi :Cytoplasm, cytoskeleton.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.