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MyoD1 Rabbit mAb [G0z4]Cat NO.: A45481

Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using MyoD1 Rabbit mAb [G0z4]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :Class C basic helix-loop-helix protein 1; MYF3; Myod 1; MYOD1; Myogenic differentiation 1; PUM;

UniProtID :P15172

MASS(da) :34,501

MW(kDa) :50kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human MyoD1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cell lysate.

Function : Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity)..

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.