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Western blot(SDS PAGE) analysis of extracts from 293T cell lysate.Using Endothelin B Receptor Rabbit mAb [6SbL]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ABCDS; Ednra; EDNRB; Endothelin B receptor precursor; ETB; ETBR; ETRB; Hirschsprung disease;
UniProtID :P24530
MASS(da) :49,644
MW(kDa) :50kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Endothelin B Receptor
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cell lysate.
Function : Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system..
Tissue specificity :Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells..
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.