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Western blot(SDS PAGE) analysis of extracts from NIH/3T3 cells.Using PDI Rabbit mAb [B29D] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PDIA2,PDIP,PDIA2_HUMAN,Protein disulfide-isomerase A2
UniProtID :Q13087
MASS(da) :58,206
MW(kDa) :57 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human PDI
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NIH/3T3 cells
Function : Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins..
Tissue specificity :Highly expressed in pancreas (at protein level)..
Subcellular locationi :Endoplasmic reticulum lumen.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.