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Western blot(SDS PAGE) analysis of extracts from (1) HeLa cell lysate; (2) Mouse brain lysate; (3) Rat brain lysate;.Using SDHA Rabbit mAb [yIhT]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PGL5;SDH1; SDH2; SDHA; SDHF;
UniProtID :P31040
MASS(da) :72,692
MW(kDa) :72kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human SDHA
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) HeLa cell lysate; (2) Mouse brain lysate; (3) Rat brain lysate;
Function : Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:24781757). Can act as a tumor suppressor (PubMed:20484225)..
Subcellular locationi :Mitochondrion inner membrane,Peripheral membrane protein,Matrix side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.