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Western blot(SDS PAGE) analysis of extracts from (1) HeLa cell lysate; (2) Mouse spleen lysate.Using FGF21 Rabbit mAb [Wy2K]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Fgf21; FGFL; Fibroblast growth factor 21; PRO10196; UNQ3115;
UniProtID :Q9NSA1
MASS(da) :22,300
MW(kDa) :22kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human FGF21
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) HeLa cell lysate; (2) Mouse spleen lysate.
Function : Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity..
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.