* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from HCT116 cells.Using PDK1 Rabbit mAb [2ICB] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PDK1,PDHK1,PDK1_HUMAN,[Pyruvate dehydrogenase ] kinase isozyme 1, mitochondrial
UniProtID :Q15118
MASS(da) :49,244
MW(kDa) :58-68 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human PDK1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HCT116 cells
Function : Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress..
Tissue specificity :Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas..
Subcellular locationi :Mitochondrion matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.