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Western blot(SDS PAGE) analysis of extracts from A375 cell lysate.Using CD73 Rabbit mAb [l7E2]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :5' nucleotidase (CD73); 5' nucleotidase precursor; 5' nucleotidase; ecto; 5NTD; CD73; CD73 antigen; E5NT; Ecto 5' nucleotidase; Ecto-5'-nucleotidase; NT5E; NTE;
UniProtID :P21589
MASS(da) :63,368
MW(kDa) :70kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human CD73
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A375 cell lysate.
Function : Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities..
Subcellular locationi :Cell membrane,Lipid-anchor, GPI-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.