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Western blot(SDS PAGE) analysis of extracts from human urine sample lysate.Using EGF Rabbit mAb [cS7F]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Beta urogastrone; Egf; Epidermal growth factor; HOMG4; Pro epidermal growth factor; URG; Urogastrone;
UniProtID :P01133
MASS(da) :133,994
MW(kDa) :160kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human EGF
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human urine sample lysate.
Function : EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941)..
Tissue specificity :Expressed in kidney, salivary gland, cerebrum and prostate..
Subcellular locationi :Membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.