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GATA3 Rabbit mAb [7Ho3]Cat NO.: A83124

Western blot(SDS PAGE) analysis of extracts from (1) SH-SY5Y cell lysate; (2) Jurkat cell lysate.Using GATA3 Rabbit mAb [7Ho3]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :GATA-binding factor 3; GATA3;

UniProtID :P23771

MASS(da) :47,916

MW(kDa) :48kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human GATA3

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :(1) SH-SY5Y cell lysate; (2) Jurkat cell lysate.

Function : Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression (By similarity)..

Tissue specificity :T-cells and endothelial cells.

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.