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Western blot(SDS PAGE) analysis of extracts from (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate.Using CRISPR-Cas9 SP Rabbit mAb [hoiT]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Cas9; CRISPR-associated endonuclease Cas9/Csn1; CRISPR-Cas9/Csn1; csn1; SpyCas9;
UniProtID :Q99ZW2
MW(kDa) :158kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Streptococcus pyogenes
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human CRISPR-Cas9 SP
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.