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Western blot(SDS PAGE) analysis of extracts from 293T cell lysate.Using CHD4 Rabbit mAb [pOnk]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ATP dependent helicase CHD4; CHD4; Chromodomain helicase DNA binding protein 4; Mi 2 autoantigen 218 kDa protein; Mi 2b; Mi2 beta;
UniProtID :Q14839
MASS(da) :218,005
MW(kDa) :260kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human CHD4
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cell lysate.
Function : Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones..
Subcellular locationi :Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.