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Puma Rabbit mAb [J57J]Cat NO.: A35731

Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using Puma Rabbit mAb [J57J] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :BBC3,PUMA,BBC3_HUMAN,Bcl-2-binding component 3, isoforms 1/2

UniProtID :Q9BXH1

MASS(da) :20,532

MW(kDa) :23 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Puma

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HepG2 cells

Function : Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis (PubMed:11463391, PubMed:23340338). Promotes partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53, releasing the bound p53/TP53 to induce apoptosis (PubMed:23340338). Regulates ER stress-induced neuronal apoptosis (By similarity)..

Tissue specificity :Ubiquitously expressed..

Subcellular locationi :Mitochondrion.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.