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Western blot(SDS PAGE) analysis of extracts from (1) HepG2 cell lysate; (2) NIH/3T3 cell lysate.Using GLUT3 Rabbit mAb [o9YV]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :brain; Glucose transporter type 3; GLUT3; Slc2a3;
UniProtID :P11169
MASS(da) :53,924
MW(kDa) :54kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human GLUT3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) HepG2 cell lysate; (2) NIH/3T3 cell lysate.
Function : Facilitative glucose transporter that can also mediate the uptake of various other monosaccharides across the cell membrane (PubMed:9477959, PubMed:26176916). Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate (PubMed:9477959, PubMed:26176916). Does not mediate fructose transport (PubMed:9477959, PubMed:26176916)..
Tissue specificity :Highly expressed in brain. Expressed in many tissues..
Subcellular locationi :Cell membrane,Multi-pass membrane protein. Perikaryon. Cell projection.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.