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Western blot(SDS PAGE) analysis of extracts from JAR cell lysate.Using PTGER2 Rabbit mAb [0fk3]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EP2; PGE2 receptor EP2 subtype; Prostaglandin E receptor 2 subtype EP2 53kDa; Prostaglandin E2 receptor EP2 subtype; Prostanoid EP2 receptor; Ptger2;
UniProtID :P43116
MASS(da) :39,761
MW(kDa) :52kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat Cow
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human PTGER2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :JAR cell lysate.
Function : Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
Tissue specificity :Placenta and lung.
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.