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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate treated with Camptothecin.Using Alpha Fodrin Rabbit mAb [GHAp]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Alpha II Spectrin; EIEE5; Fodrin alpha chain; NEAS; SPECA; Spectrin; Spna2; SPTAN1;
UniProtID :Q13813
MASS(da) :284,539
MW(kDa) :150,285kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Alpha Fodrin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate treated with Camptothecin.
Function : Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Subcellular locationi :Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.