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Western blot(SDS PAGE) analysis of extracts from MCF-7 cells.Using Rab25 Rabbit mAb [FQB4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RAB25,CATX8,RAB25_HUMAN,Ras-related protein Rab-25
UniProtID :P57735
MASS(da) :23,496
MW(kDa) :23 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Rab25
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells
Function : Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226). Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (By similarity). May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis (By similarity)..
Tissue specificity :Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer,expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer,expression is increased relative to normal breast tissue..
Subcellular locationi :Cell membrane,Lipid-anchor,Cytoplasmic side. Cell projection, pseudopodium membrane. Cytoplasmic vesicle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.