TXNRD1 Rabbit mAb [SjzY]Cat NO.: A10224

Protein names ：GRIM12; KDRF; KM 102 derived reductase like factor; xidoreductase; Thioredoxin reductase 1; TR1; TRXR1; TXNR; TXNRD1

UniProtID ：Q16881

MASS(da) ：70,906

MW(kDa) ：55kDa

Form ：Liquid

Purification ：Affinity-chromatography

Host ：Rabbit

Isotype ： IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with A synthesized peptide derived from human TXNRD1

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：(1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.

Function ： Reduces disulfideprotein thioredoxin (Trx) to its dithiol-containing form (PubMed:8577704). Homodimeric flavoprotein involved in the regulation of cellular redox reactions, growth and differentiation. Contains a selenocysteine residue at the C-terminal active site that is essential for catalysis (Probable). Also has reductase activity on hydrogen peroxide (H2O2) (PubMed:10849437).., [Isoform 1]: Induces actin and tubulin polymerization, leading to formation of cell membrane protrusions.., [Isoform 4]: Enhances the transcriptional activity of estrogen receptors ESR1 and ESR2.., [Isoform 5]: Enhances the transcriptional activity of the estrogen receptor ESR2 only (PubMed:15199063). Mediates cell death induced by a combination of interferon-beta and retinoic acid (PubMed:9774665)..

Tissue specificity ：[Isoform 1]: Expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines..,[Isoform 4]: Widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver..

Subcellular locationi ：[Isoform 1]: Cytoplasm.,[Isoform 4]: Cytoplasm. Nucleus.,[Isoform 5]: Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.